675502

antibody from BioLegend

Targeting: MAPK1 ERK, ERK2, MAPK2, p41mapk, PRKM1, PRKM2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

675502

Provider

BioLegend

Proper citation

BioLegend Cat#675502, RRID:AB_2565604

Product name

Purified anti-ERK1/2 Phospho (Thr202/Tyr204)

Antibody type

Monoclonal

Antigen

Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH.

Description

ERK1/2 are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

4B11B69

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Untreated (lane 1) and PMA-treated (lane 2) Jurkat whole cell lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-ERK1/2 Phospho (Thr202/Tyr204) (clone 4B11B69) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP (Cat. No. 405306) and chemiluminescence detection.

Conjugate

Unconjugated