TA324912

antibody from OriGene

Targeting: MAPK1 ERK, ERK2, MAPK2, p41mapk, PRKM1, PRKM2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324912

Provider

OriGene

Product name

Rabbit polyclonal MAPK1 Antibody (Center)

Antibody type

Polyclonal

Description

Rabbit polyclonal MAPK1 Antibody (Center)

Host

Rabbit

Conjugate

Unconjugated

Epitope

MAPK1

Isotype

IgG

Antibody clone number

NULL

Vial size

400 µl

Concentration

2.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of anti-MAPK1 Antibody (Center) Pab (RB11609) in Hela, 293, and HepG2 cell line lysates. MAPK1 Antibody (Center)(arrow) was detected using the purified Pab.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with MAPK1 Antibody (Center)(Cat.#TA324912), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

MAPK1 Antibody (Center) (Cat. #TA324912) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Validation comment

FC