AM00085PU-N

antibody from Acris Antibodies GmbH

Targeting: MAPK1 ERK, ERK2, MAPK2, p41mapk, PRKM1, PRKM2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

AM00085PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00085PU-N, RRID:AB_981012

Product name

anti ERK2 (C-term) (incl. pos. control)

Antibody type

Monoclonal

Antigen

Peptide conjugated to KLHEpitope: C-terminus

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

6G11

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Detection of endogenous MAPK2: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab MAPK2-6G11 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: A549 Lane 3: SKOV3 Lane 4: OVCAR5 Lane 5: HaCaT lane 6: PC3 Lane 7: HeLaLane 8: HepG2