Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- R30228 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MAPK1/3 Antibody / ERK1/2
- Antibody type
- Polyclonal
- Description
- This highly specific MAPK1/3 antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the MAPK1/3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of MAPK1/3 antibody and HeLa cell lysate
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human K562, 3) human A431, 4) human U-2 OS, 5) rat brain, 6) rat C6, 7) mouse brain and 8) mouse Neuro-2a cell lysate with MAPK1/3 antibody. Predicted molecular weight ~41 kDa (MAPK1) and ~36 kDa (MAPK3).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with MAPK1/3 antibody (green) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: MAPK1/3 antibody testing of human breast cancer tissue
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human HeLa cells with MAPK1/3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MAPK1/3 antibody.
