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ImmunocytochemistryAntibody data
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- Antigen structure
- References [8]
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- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- GTX24819 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX24819, RRID:AB_380440
- Product name
- ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Drosophila, Xenopus
- Host
- Rabbit
Submitted references Effects of hypothermia during propofol anesthesia on learning and memory ability and hippocampal apoptosis in neonatal rats.
Upregulation of LGALS1 is associated with oral cancer metastasis.
Enhanced Capability of Bone Morphogenetic Protein 2-loaded Mesoporous Calcium Silicate Scaffolds to Induce Odontogenic Differentiation of Human Dental Pulp Cells.
18β-glycyrrhetinic acid inhibits migration and invasion of human gastric cancer cells via the ROS/PKC-α/ERK pathway.
The effects of artocarpin on wound healing: in vitro and in vivo studies.
The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages.
Mycobacterium tuberculosis Upregulates TNF-α Expression via TLR2/ERK Signaling and Induces MMP-1 and MMP-9 Production in Human Pleural Mesothelial Cells.
Erythropoietin protects neuroblastoma cells against etoposide and vincristine by activating ERK and AKT pathways but has no effect in kidney cells.
Liu W, Tan X, Xiong X, Yang J, Xiao X
Journal of anesthesia 2019 Feb;33(1):9-16
Journal of anesthesia 2019 Feb;33(1):9-16
Upregulation of LGALS1 is associated with oral cancer metastasis.
Li JM, Tseng CW, Lin CC, Law CH, Chien YA, Kuo WH, Chou HC, Wang WC, Chan HL
Therapeutic advances in medical oncology 2018;10:1758835918794622
Therapeutic advances in medical oncology 2018;10:1758835918794622
Enhanced Capability of Bone Morphogenetic Protein 2-loaded Mesoporous Calcium Silicate Scaffolds to Induce Odontogenic Differentiation of Human Dental Pulp Cells.
Huang KH, Chen YW, Wang CY, Lin YH, Wu YA, Shie MY, Lin CP
Journal of endodontics 2018 Nov;44(11):1677-1685
Journal of endodontics 2018 Nov;44(11):1677-1685
18β-glycyrrhetinic acid inhibits migration and invasion of human gastric cancer cells via the ROS/PKC-α/ERK pathway.
Cai H, Chen X, Zhang J, Wang J
Journal of natural medicines 2018 Jan;72(1):252-259
Journal of natural medicines 2018 Jan;72(1):252-259
The effects of artocarpin on wound healing: in vitro and in vivo studies.
Yeh CJ, Chen CC, Leu YL, Lin MW, Chiu MM, Wang SH
Scientific reports 2017 Nov 15;7(1):15599
Scientific reports 2017 Nov 15;7(1):15599
The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages.
Chen CC, Lin MW, Liang CJ, Wang SH
PloS one 2016;11(7):e0158662
PloS one 2016;11(7):e0158662
Mycobacterium tuberculosis Upregulates TNF-α Expression via TLR2/ERK Signaling and Induces MMP-1 and MMP-9 Production in Human Pleural Mesothelial Cells.
Chen WL, Sheu JR, Chen RJ, Hsiao SH, Hsiao CJ, Chou YC, Chung CL, Hsiao G
PloS one 2015;10(9):e0137979
PloS one 2015;10(9):e0137979
Erythropoietin protects neuroblastoma cells against etoposide and vincristine by activating ERK and AKT pathways but has no effect in kidney cells.
Vazquez-Mellado MJ, Aguilar C, Rocha-Zavaleta L
Life sciences 2015 Sep 15;137:142-9
Life sciences 2015 Sep 15;137:142-9
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Extracts prepared from PC12 cells left unstimulated (1) or stimulated with sorbitol (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4 degree C then incubated with GTX24819
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NIH3T3 or NIH3T3-L1 cells were treated with either PDGF or Insulin. Western Blots were performed using either Akt [pS473] or Erk [pTpY185/187] (GTX24819) primary antibodies. Anti-rabbit secondary antibody conjugated to Alexa fluor 680 was used for detection. Akt [pS473]: Lane 1 ¡V NIH3T3 control, 20 ?g Lane 2 ¡V NIH3T3 + PDGF, 20 ?g Lane 3 ¡V NIH3T3-L1 control, 20 ?g Lane 4 ¡V NIH3T3-L1 + Insulin, 20 ?g. ERK [pTpY185/187]: Lane 6 ¡V NIH3T3 control, 5 ?g Lane 7 ¡V NIH3T3 + PDGF, 5 ?g
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of MDA-MB-231 with treatment of EGF(100ng/ml for 15mins) (Lane 1), MDA-MB-231 (Lane 2), U-87 MG (Lane 3), SH-SY5Y (Lane 4) and HeLa (Lane 5) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.Dilution : 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of human breast carcinoma tissue (right) compared to a negative control without primary antibody (left) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.Antigen retrieval : 10mM sodium citrate (pH 6.0)Dilution : 1:50
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of human colon carcinoma (right) compared to a negative control without primary antibody (left) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.Antigen retrieval : 10mM sodium citrate (pH 6.0)Dilution : 1:20
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of mouse stomach tissue (right) compared to a negative control without primary antibody (left) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.Antigen retrieval : 10mM sodium citrate (pH 6.0)Dilution : 1:20
