ARG10585

antibody from Arigo

Targeting: MAPK1 ERK, ERK2, MAPK2, p41mapk, PRKM1, PRKM2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ARG10585

Provider

Arigo

Product name

anti-Erk1/2 phospho (Thr185/Tyr187) antibody [G15-B]

Antibody type

Monoclonal

Antigen

Phosphospecific peptide pT-E-pY motif of activated Erk 1,2

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

G15-B

Vial size

50 µl

Storage

For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 50 µg of A) untreated PC12 cells, B) cells treated with EGF at 100 ng/ml, 5 min, C) PMA at 100nM, 30 min, D) in serum free DMEM, and EGF at 100 ng/ml, 5min with 10% FBS in DMEM stained with ARG10585 anti-Erk1/2 phospho (Thr185/Tyr187) antibody [G15-B].

Immunocytochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunocytochemistry: hydrogen peroxide treated HEK293 cells stained with ARG10585 anti-Erk1/2 phospho (Thr185/Tyr187) antibody [G15-B] at 1:100 dilution.

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunocytochemistry: HEK293 cells stained with ARG10585 anti-Erk1/2 phospho (Thr185/Tyr187) antibody [G15-B] at 1:100 dilution.