Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
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Validation data
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- Product number
- ARG10585 - Provider product page
- Provider
- Arigo
- Product name
- anti-Erk1/2 phospho (Thr185/Tyr187) antibody [G15-B]
- Antibody type
- Monoclonal
- Antigen
- Phosphospecific peptide pT-E-pY motif of activated Erk 1,2
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- G15-B
- Vial size
- 50 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 50 µg of A) untreated PC12 cells, B) cells treated with EGF at 100 ng/ml, 5 min, C) PMA at 100nM, 30 min, D) in serum free DMEM, and EGF at 100 ng/ml, 5min with 10% FBS in DMEM stained with ARG10585 anti-Erk1/2 phospho (Thr185/Tyr187) antibody [G15-B].
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunocytochemistry: hydrogen peroxide treated HEK293 cells stained with ARG10585 anti-Erk1/2 phospho (Thr185/Tyr187) antibody [G15-B] at 1:100 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunocytochemistry: HEK293 cells stained with ARG10585 anti-Erk1/2 phospho (Thr185/Tyr187) antibody [G15-B] at 1:100 dilution.