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- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- AM09368PU-S - Provider product page
- Provider
- OriGene
- Product name
- ERK2 (MAPK1) mouse monoclonal antibody, clone AT1A4, Purified
- Antibody type
- Monoclonal
- Description
- ERK2 (MAPK1) mouse monoclonal antibody, clone AT1A4, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MAPK1
- Isotype
- IgG
- Antibody clone number
- AT1A4
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Cell lysates of HepG2 (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK1 (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis: Cell lysates of HepG2 (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK1 (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence of human MCF7 cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human MAPK1 antibody (1:500) with Alexa 488 (Green).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence of human MCF7 cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human MAPK1 antibody (1:500) with Alexa 488 (Green).
- Validation comment
- IF
