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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB9958 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9958, RRID:AB_1676825
- Product name
- MAPK1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against partial recombinant MAPK1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The role of transient ERK2 signals in fibronectin- and insulin-mediated DNA synthesis.
The MAP kinase ERK2 inhibits the cyclic AMP-specific phosphodiesterase HSPDE4D3 by phosphorylating it at Ser579.
Organization and regulation of mitogen-activated protein kinase signaling pathways.
Asthagiri AR, Reinhart CA, Horwitz AF, Lauffenburger DA
Journal of cell science 2000 Dec;113 Pt 24:4499-510
Journal of cell science 2000 Dec;113 Pt 24:4499-510
The MAP kinase ERK2 inhibits the cyclic AMP-specific phosphodiesterase HSPDE4D3 by phosphorylating it at Ser579.
Hoffmann R, Baillie GS, MacKenzie SJ, Yarwood SJ, Houslay MD
The EMBO journal 1999 Feb 15;18(4):893-903
The EMBO journal 1999 Feb 15;18(4):893-903
Organization and regulation of mitogen-activated protein kinase signaling pathways.
Garrington TP, Johnson GL
Current opinion in cell biology 1999 Apr;11(2):211-8
Current opinion in cell biology 1999 Apr;11(2):211-8
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- Experimental details
- Western blot using MAPK1 polyclonal antibody (Cat # PAB9958) shows detection of MAPK1 in several whole cell lysates : HeLa (lane 1), A-431 (lane 2), and NIH/3T3 (lane 3).Detection occurs using a 1 : 1,000 dilution of the primary antibody followed by 1 : 4,000 dilution of HRP Goat-a-Rabbit with visualization via ECL.Film exposure was approximately 1'.
