TA500470

antibody from OriGene

Targeting: MAPK1 ERK, ERK2, MAPK2, p41mapk, PRKM1, PRKM2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500470

Provider

OriGene

Proper citation

OriGene Cat#TA500470, RRID:AB_10544029

Product name

Anti-MAPK1 (ERK2) mouse monoclonal antibody, clone OTI1D3 (formerly 1D3)

Antibody type

Monoclonal

Description

Anti-MAPK1 (ERK2) mouse monoclonal antibody, clone OTI1D3 (formerly 1D3)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

MAPK1

Isotype

IgG

Antibody clone number

OTI1D3

Vial size

100 µl

Concentration

0.7 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (RC204703, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAPK1 monoclonal antibody.

Validation comment

WB

Immunoprecipitation

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunoprecipitation of MAPK1 by using TrueMab monoclonal anti-MAPK1 antibody (Negative control: IP without adding anti-MAPK1 antibody). For each experiment, 500ul of DDK tagged MAPK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of ant

Validation comment

IP