TA500473

antibody from OriGene

Targeting: MAPK1 ERK, ERK2, MAPK2, p41mapk, PRKM1, PRKM2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500473

Provider

OriGene

Proper citation

OriGene Cat#TA500473, RRID:AB_10544079

Product name

Anti-MAPK1 (ERK2) mouse monoclonal antibody, clone OTI7C9 (formerly 7C9)

Antibody type

Monoclonal

Description

Anti-MAPK1 (ERK2) mouse monoclonal antibody, clone OTI7C9 (formerly 7C9)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

MAPK1

Isotype

IgG

Antibody clone number

OTI7C9

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (RC204703, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAPK1 monoclonal antibody.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-MAPK1 mouse monoclonal antibody (TA500473) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK1(RC204703).

Validation comment

IF

Immunoprecipitation

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunoprecipitation of MAPK1 by using TrueMab monoclonal anti-MAPK1 antibody (Negative control: IP without adding anti-MAPK1 antibody). For each experiment, 500ul of DDK tagged MAPK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of ant

Validation comment

IP