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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
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- Product number
- TA500486 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500486, RRID:AB_2141147
- Product name
- Anti-MAPK1 (ERK2) mouse monoclonal antibody, clone OTI7E7 (formerly 7E7)
- Antibody type
- Monoclonal
- Description
- Anti-MAPK1 (ERK2) mouse monoclonal antibody, clone OTI7E7 (formerly 7E7)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MAPK1
- Isotype
- IgG
- Antibody clone number
- OTI7E7
- Vial size
- 100 µl
- Concentration
- 0.85 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAPK1 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (RC204703, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of COS7 cells using anti-MAPK1 mouse monoclonal antibody (TA500486).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-MAPK1 mouse monoclonal antibody (TA500486) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK1(RC204703).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of MAPK1 by using TrueMab monoclonal anti-MAPK1 antibodies (Negative control: IP without adding anti-MAPK1 antibody.). For each experiment, 500ul of DDK tagged MAPK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAPK1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Validation comment
- IP
