Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN359631 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ERK2.
- Description
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Regulation of the activity of p38 mitogen-activated protein kinase by Akt in cancer and adenoviral protein E1A-mediated sensitization to apoptosis.
Parallel phosphatidylinositol-3 kinase and p42/44 mitogen-activated protein kinase signaling pathways subserve the mitogenic and antiapoptotic actions of insulin-like growth factor I in osteoblastic cells.
Cyclosporin A induces erythroid differentiation of K562 cells through p38 MAPK and ERK pathways.
Different regulation of PKC isoenzymes and MAPK by PSK and IL-2 in the proliferative and cytotoxic activities of the NKL human natural killer cell line.
Persistent activation of ERK1/2 by lead acetate increases nucleotide excision repair synthesis and confers anti-cytotoxicity and anti-mutagenicity.
Liao Y, Hung MC
Molecular and cellular biology 2003 Oct;23(19):6836-48
Molecular and cellular biology 2003 Oct;23(19):6836-48
Parallel phosphatidylinositol-3 kinase and p42/44 mitogen-activated protein kinase signaling pathways subserve the mitogenic and antiapoptotic actions of insulin-like growth factor I in osteoblastic cells.
Grey A, Chen Q, Xu X, Callon K, Cornish J
Endocrinology 2003 Nov;144(11):4886-93
Endocrinology 2003 Nov;144(11):4886-93
Cyclosporin A induces erythroid differentiation of K562 cells through p38 MAPK and ERK pathways.
Sawafuji K, Miyakawa Y, Kizaki M, Ikeda Y
American journal of hematology 2003 Jan;72(1):67-9
American journal of hematology 2003 Jan;72(1):67-9
Different regulation of PKC isoenzymes and MAPK by PSK and IL-2 in the proliferative and cytotoxic activities of the NKL human natural killer cell line.
GarcĂa-Lora A, Martinez M, Pedrinaci S, Garrido F
Cancer immunology, immunotherapy : CII 2003 Jan;52(1):59-64
Cancer immunology, immunotherapy : CII 2003 Jan;52(1):59-64
Persistent activation of ERK1/2 by lead acetate increases nucleotide excision repair synthesis and confers anti-cytotoxicity and anti-mutagenicity.
Lin YW, Chuang SM, Yang JL
Carcinogenesis 2003 Jan;24(1):53-61
Carcinogenesis 2003 Jan;24(1):53-61
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of anti-ERK2 C-term Pab in UV-treated HL-60 cell lysate. ERK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ERK2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.