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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-19102 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-19102, RRID:AB_1641990
- Product name
- Rabbit Polyclonal ERK2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ERK2 Antibody [NBP1-19102] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50ug) cells. Antibody used at for WB at 0.1 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ERK2 Antibody [NBP1-19102] - Samples: 1 mg whole cell lysate was Immunoprecipitated from HeLa cells that were mock treated (-) or treated with TPA (+); 20% of the IP was loaded. Antibodies: In (A), (B), and (C), affinity purified goat anti-Phospho ERK1/2 NBP1-97749 was used for IP (6ug/1mg lysate). In (A) Rabbit anti-ERK1 (NBP1-19101) was used for western blot (WB) at 1 ug/ml. In (B) Rabbit anti-ERK2 (NBP1-19102) was used for (WB) at 1ug/ml. In (C) a mix of anti-ERK1 (NBP1-19101) and anti-ERK2 (NBP1-19102) was used for WB at 1ug/ml each. Detection: Chemiluminescence with exposure times of 30 seconds for (A), (B), and (C).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-19102] - Human breast carcinoma. Antibody used at a dilution of 1:1,000.
