PA5-34574

antibody from Invitrogen Antibodies

Targeting: FGFR3 ACH, CD333, CEK2, JTK4

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34574

Provider

Invitrogen Antibodies

Product name

FGFR3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is SK-N-SH cell lysate. PA5-34574 can be used with blocking peptide PEP-1617.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C

References

Submitted references

Neuron-Derived Estrogen Is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain.

Lu Y, Sareddy GR, Wang J, Zhang Q, Tang FL, Pratap UP, Tekmal RR, Vadlamudi RK, Brann DW
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Sep 16;40(38):7355-7374

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The receptor protein tyrosine phosphatase PTPRB negatively regulates FGF2-dependent branching morphogenesis.

Soady KJ, Tornillo G, Kendrick H, Meniel V, Olijnyk-Dallis D, Morris JS, Stein T, Gusterson BA, Isacke CM, Smalley MJ
Development (Cambridge, England) 2017 Oct 15;144(20):3777-3788

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 9. Evidence that upregulation of neuronal FGF2 signaling mediates decreased astrocyte activation in ovariectomized female FBN-ARO-KO mice after GCI. Aa , Representative image of FGF2 and NeuN double staining in hippocampal CA1 region. Ab , Quantitative analysis of neuronal FGF2 levels in each group ( N = 4 or 5). B , Neuronal FGF2 alterations in hippocampus were confirmed by Western blotting analysis ( N = 3). C , Representative images of FGFR3 and GFAP double staining showing changes of FGFR3 expression in hippocampal CA1 astrocytes ( N = 4 or 5). Da , Schematic illustration of FGFR3 neutralization by bilateral intracerebroventricular injection of FGFR3 blocking antibody in FBN-ARO-KO GCI mice. Db , The purity of isolated astrocytes from the ischemic brains was evaluated by flow cytometry analysis. E , To examine the effects of FGFR3 neutralization on astrocyte reactivity and functional restoration in FBN-ARO-KO mice after GCI, levels of GFAP, p-STAT3, BDNF, and GLT-1 in purified astrocytes were determined by Western blotting ( Ea ), and further quantitatively analyzed ( Eb ) ( N = 3). Fa , IHC analysis for neuronal degeneration by F-Jade C and NeuN double staining in hippocampal CA1 region. Fb , Quantification of F-Jade C levels in CA1 pyramidal neurons, which was presented as relative intensity of each group versus FLOX+GCI ( N = 4 or 5). Values are mean +- SEM of determinations from each group. * p < 0.05 versus FLOX+Sham. # p < 0.05 versus FLOX+GCI. $ p < 0.05 ver

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 10. Exogenous E2 replacement decreases hippocampal FGF2 signaling in ovariectomized female FBN-ARO-KO mice. Aa , Effect of exogenous E2 replacement on neuronal FGF2 expression in FBN-ARO-KO mice was examined by NeuN and FGF2 double staining. Ab , Relative neuronal FGF2 intensities after E2 treatment were quantitatively analyzed. Ba , Levels of the major FGF2 receptor FGFR3 in hippocampal astrocytes were determined by GFAP and FGFR3 staining. Bb , FGFR3 relative intensities in FBN-ARO-KO mice after E2 rescue were quantified. Values are mean +- SEM of determinations from each group. N = 4. * p < 0.05 versus FLOX+GCI. # p < 0.05 versus KO+GCI.