Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX100722 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100722, RRID:AB_1950485
- Product name
- HDAC6 antibody [N1], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Inhibition of HDAC3- and HDAC6-Promoted Survivin Expression Plays an Important Role in SAHA-Induced Autophagy and Viability Reduction in Breast Cancer Cells.
A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.
Histone deacetylase 6 regulates estrogen receptor alpha in uterine leiomyoma.
Lee JY, Kuo CW, Tsai SL, Cheng SM, Chen SH, Chan HH, Lin CH, Lin KY, Li CF, Kanwar JR, Leung EY, Cheung CC, Huang WJ, Wang YC, Cheung CH
Frontiers in pharmacology 2016;7:81
Frontiers in pharmacology 2016;7:81
A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.
Lee KH, Lo HL, Tang WC, Hsiao HH, Yang PM
Scientific reports 2014 Sep 17;4:6394
Scientific reports 2014 Sep 17;4:6394
Histone deacetylase 6 regulates estrogen receptor alpha in uterine leiomyoma.
Wei LH, Torng PL, Hsiao SM, Jeng YM, Chen MW, Chen CA
Reproductive sciences (Thousand Oaks, Calif.) 2011 Aug;18(8):755-62
Reproductive sciences (Thousand Oaks, Calif.) 2011 Aug;18(8):755-62
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE GTX100722 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HDAC6 antibody detects HDAC6 protein by western blot analysis. Non-transfected (-) and HDAC6-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with HDAC6 antibody (GTX100722) diluted by 1:5000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using HDAC6(GTX100722) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.