Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- GTX21440 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX21440, RRID:AB_371576
- Product name
- HDAC6 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Sam68 Allows Selective Targeting of Human Cancer Stem Cells.
Benoit YD, Mitchell RR, RisueƱo RM, Orlando L, Tanasijevic B, Boyd AL, Aslostovar L, Salci KR, Shapovalova Z, Russell J, Eguchi M, Golubeva D, Graham M, Xenocostas A, Trus MR, Foley R, Leber B, Collins TJ, Bhatia M
Cell chemical biology 2017 Jul 20;24(7):833-844.e9
Cell chemical biology 2017 Jul 20;24(7):833-844.e9
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sonicated Chromatin prepared from untreated (UI) or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with GTX21440 to HDAC6 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are nomalized over inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 10 ul of GTX21440 and 2x10*6 cells were used in each ChIP experiment
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of HDAC6 using GTX21440 in Jurkat cell lysate