PA5-38498
antibody from Invitrogen Antibodies
Targeting: HDAC6
FLJ16239, HD6, JM21, KIAA0901, PPP1R90
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-38498 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-HDAC6 (Ser22) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-HDAC6 pSer22 in extracts from NIH-3T3 cells using a Phospho-HDAC6 pSer22 polyclonal antibody (Product # PA5-38498).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-HDAC6 pSer22 in HepG2 cells using a Phospho-HDAC6 pSer22 polyclonal antibody (Product # PA5-38498).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-HDAC6 pSer22 in HepG2 cells using a Phospho-HDAC6 pSer22 polyclonal antibody (Product # PA5-38498).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Phospho-HDAC6 (Ser22) was performed using 70% confluent log phase A549 cells treated with EGF (50 ng/mL for 10 min). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-HDAC6 (Ser22) Polyclonal Antibody (Product # PA5-38498) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing increased expression of nuclear signal upon EGF treatment. Panel e represents the control cells showing weak nuclear staining. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-HDAC6 pSer22 in paraffin-embedded human testis tissue using a Phospho-HDAC6 pSer22 polyclonal antibody (Product # PA5-38498).