H00010013-M01

antibody from Abnova Corporation

Targeting: HDAC6 FLJ16239, HD6, JM21, KIAA0901, PPP1R90

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00010013-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00010013-M01, RRID:AB_464293

Product name

HDAC6 monoclonal antibody (M01), clone 1E2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant HDAC6.

Antigen sequence

DVTQPCGDCGTIQENWVCLSCYQVYCGRYINGHML
QHHGNSGHPLVLSYIDLSAWCYYCQAYVHHQALLD
VKNIAHQNKFGEDMPHPH


Isotype

IgG

Antibody clone number

1E2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes.

Bantscheff M, Hopf C, Savitski MM, Dittmann A, Grandi P, Michon AM, Schlegl J, Abraham Y, Becher I, Bergamini G, Boesche M, Delling M, Dümpelfeld B, Eberhard D, Huthmacher C, Mathieson T, Poeckel D, Reader V, Strunk K, Sweetman G, Kruse U, Neubauer G, Ramsden NG, Drewes G
Nature biotechnology 2011 Mar;29(3):255-65

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

HDAC6 monoclonal antibody (M01), clone 1E2 Western Blot analysis of HDAC6 expression in Hela S3 NE ( Cat # L013V3 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HDAC6 expression in transfected 293T cell line by HDAC6 monoclonal antibody (M01), clone 1E2.Lane 1: HDAC6 transfected lysate (Predicted MW: 131.4 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged HDAC6 is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol