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LearnMA5-25317
antibody from Invitrogen Antibodies
Targeting: HDAC6
FLJ16239, HD6, JM21, KIAA0901, PPP1R90
Western blot
Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [5]
- Immunohistochemistry [4]
- Chromatin Immunoprecipitation [2]
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- Product number
- MA5-25317 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HDAC6 Monoclonal Antibody (OTI4C5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI4C5
- Vial size
- 100 μL
- Concentration
- 1 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HDAC6 in COS7 cells. Cells were transfected with a plasmid overexpressing HDAC6 and probed with a HDAC6 monoclonal antibody (Product # MA5-25317).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HDAC6 in COS7 cells. Cells were transfected with a plasmid overexpressing HDAC6 and probed with a HDAC6 monoclonal antibody (Product # MA5-25317).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HDAC6 in COS7 cells. Cells were transfected with a plasmid overexpressing HDAC6 and probed with a HDAC6 monoclonal antibody (Product # MA5-25317).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HDAC6 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HDAC6 Mouse Monoclonal Antibody (Product # MA5-23517) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing nuclear and cytoplasmic localization of HDAC6. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HDAC6 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HDAC6 Mouse Monoclonal Antibody (Product # MA5-23517) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing nuclear and cytoplasmic localization of HDAC6. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HDAC6 in paraffin-embedded adenocarcinoma of human ovary tissue using a HDAC6 monoclonal antibody (Product # MA5-25317) at a dilution of 1:150.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HDAC6 in paraffin-embedded human liver tissue using a HDAC6 monoclonal antibody (Product # MA5-25317) at a dilution of 1:150.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HDAC6 in paraffin-embedded human colon tissue using a HDAC6 monoclonal antibody (Product # MA5-25317) at a dilution of 1:150.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HDAC6 in paraffin-embedded human kidney tissue using a HDAC6 monoclonal antibody (Product # MA5-25317) at a dilution of 1:150.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP) assay of endogenous HDAC6 protein using Anti-HDAC6 Antibody: ChIP was performed using Anti-HDAC6 Mouse Monoclonal Antibody (Product # MA5-25317, 5 µg) on sheared chromatin from HeLa cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Mouse IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to IGFBP4 promoter (-4.2kb), SMARCA2 transcriptional start site, AXIN2 exon1 and GAPDH promoter. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP) assay of endogenous HDAC6 protein using Anti-HDAC6 Antibody: ChIP was performed using Anti-HDAC6 Mouse Monoclonal Antibody (Product # MA5-25317, 5 µg) on sheared chromatin from HeLa cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Mouse IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to IGFBP4 promoter (-4.2kb), SMARCA2 transcriptional start site, AXIN2 exon1 and GAPDH promoter. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
