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LearnMA5-25326
antibody from Invitrogen Antibodies
Targeting: HDAC6
FLJ16239, HD6, JM21, KIAA0901, PPP1R90
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
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- Product number
- MA5-25326 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HDAC6 Monoclonal Antibody (OTI1D11)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI1D11
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HDAC6 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with HDAC6 (Product # MA5-25326) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HDAC6 in HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells using 35 µg per lane. Samples were probed with HDAC6 (Product # MA5-25326) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of HDAC6 was achieved by transfecting A549 with HDAC6 specific siRNAs (Silencer® select Product # s19459). Western blot analysis (Fig. a) was performed using whole cell extracts from the HDAC6 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with HDAC6 Monoclonal Antibody (OTI1D11) (Product # MA5-25326, 1:1000 dilution) and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to HDAC6.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-HDAC6 Monoclonal Antibody (OTI1D11) (Product # MA5-25326) and a 160kDa band corresponding to HDAC6 was observed in the cell lines tested. Whole cell extracts of A549 (Lane 1), HeLa (Lane 2), MCF7 (Lane 3) and HT-29 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HDAC6 in COS7 cells. Cells were transfected with a plasmid overexpressing HDAC6 and probed with a HDAC6 monoclonal antibody (Product # MA5-25326).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HDAC6 in COS7 cells. Cells were transfected with a plasmid overexpressing HDAC6 and probed with a HDAC6 monoclonal antibody (Product # MA5-25326).
