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LearnAPRab04766
antibody from EnkiLife Biotech Co., Ltd.
Targeting: HDAC6
FLJ16239, HD6, JM21, KIAA0901, PPP1R90
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- APRab04766 - Provider product page
- Provider
- EnkiLife Biotech Co., Ltd.
- Product name
- HDAC6 (phospho Ser22) Rabbit Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- Affinity purification
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- Polyclonal
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Western blot analysis of lysates from NIH/3T3 cells treated with Anisomycin 25ug/ml 30‘, using HDAC6 （Phospho-Ser22） Antibody. The lane on the right is blocked with the phospho peptide.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Western Blot analysis of 3T3 cells using Phospho-HDAC6 （S22） Polyclonal Antibody diluted at 1：500
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using HDAC6 （Phospho-Ser22） Antibody
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HepG2 cells, using HDAC6 （Phospho-Ser22） Antibody. The picture on the right is blocked with the phospho peptide.
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded human testis, using HDAC6 （Phospho-Ser22） Antibody. The picture on the right is blocked with the phospho peptide.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.
