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antibody from Boster Biological Technology
Targeting: LAMC2
BM600-100kDa, EBR2, EBR2A, kalinin-105kDa, LAMB2T, LAMNB2, nicein-100kDa
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- PA1582 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Laminin subunit gamma-2 LAMC2 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for LAMC2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. LAMC2 information: Molecular Weight: 130976 MW; Subcellular Localization: Secreted, extracellular space, extracellular matrix, basement membrane. Major component; Tissue Specificity: The large variant is expressed only in specific epithelial cells of embryonic and neonatal tissues. In 17-week old embryo the small variant is found in cerebral cortex, lung, and distal tubes of kidney, but not in epithelia except for distal tubuli.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysisof LAMC2 using anti- LAMC2 antibody (PA1582). LAMC2 was detected in paraffin-embedded section of human lung cancertissue. Heat mediated antigen retrieval was performed inEDTA buffer (pH8.0, epitope retrieval solution). Thetissue section was blocked with 10% goat serum. The tissue sectionwasthen incubated with 1μg/ml rabbit anti-LAMC2 Antibody (PA1582) overnight at 4°C. Biotinylated goat anti-rabbitIgG was used assecondary antibody and incubated for 30 minutes at 37°C. The tissuesection was developed using Strepavidin-Biotin-Complex (SABC)(Catalog# SA1022) with DAB as the chromogen.
- Additional image
