Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB864 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal PDK-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat PDK-1 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references PDK1 Deficit Impairs the Development of the Dentate Gyrus in Mice.
Fatty acid-binding protein 5 (FABP5) regulates cognitive function both by decreasing anandamide levels and by activating the nuclear receptor peroxisome proliferator-activated receptor β/δ (PPARβ/δ) in the brain.
Xu M, Han X, Liu R, Li Y, Qi C, Yang Z, Zhao C, Gao J
Cerebral cortex (New York, N.Y. : 1991) 2019 Mar 1;29(3):1185-1198
Cerebral cortex (New York, N.Y. : 1991) 2019 Mar 1;29(3):1185-1198
Fatty acid-binding protein 5 (FABP5) regulates cognitive function both by decreasing anandamide levels and by activating the nuclear receptor peroxisome proliferator-activated receptor β/δ (PPARβ/δ) in the brain.
Yu S, Levi L, Casadesus G, Kunos G, Noy N
The Journal of biological chemistry 2014 May 2;289(18):12748-58
The Journal of biological chemistry 2014 May 2;289(18):12748-58
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat PDK-1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, Daudi human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and C6 rat glioma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PDK-1 Monoclonal Antibody (Catalog # MAB864) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PDK-1 at approximately 58-68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.