PA5-36071

antibody from Invitrogen Antibodies

Targeting: PDPK1 PDK1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36071

Provider

Invitrogen Antibodies

Product name

PDPK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 63 kDa.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PDPK1 in Lane 1: MCF-7 whole cell lysate (40 µg), Lane 2: A549 whole cell lysate (40 µg), Lane 3: PC12 whole cell lysate (40 µg), Lane 4: 3T3-L1 whole cell lysate (40 µg). Samples were incubated with PDPK1 polyclonal antibody (Product # PA5-36071) at a dilution of 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PDPK1 in Lane 1: HCC827 whole cell lysate (40 µg), Lane 2: A549 whole cell lysate (40 µg), Lane 3: NIH-3T3 whole cell lysate (40 µg), Lane 4: the Lung tissue lysate of rat (40 µg). Samples were incubated with PDPK1 polyclonal antibody (Product # PA5-36071) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PDPK1 in Lane 1: MCF-7 whole cell lysate (40 µg), Lane 2: A549 whole cell lysate (40 µg), Lane 3: PC12 whole cell lysate (40 µg), Lane 4: 3T3-L1 whole cell lysate (40 µg). Samples were incubated with PDPK1 polyclonal antibody (Product # PA5-36071) at a dilution of 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PDK1 using PDK1 polyclonal antibody (Product # PA5-36071) at a dilution of 1:500. Lane 1: A549 whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: Rat brain tissue lysate.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PDPK1 in paraffin-embedded human breast carcinoma tissue. Samples were incubated with PDPK1 polyclonal antibody (Product # PA5-36071) at a dilution of 1:100.