Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA319161 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-PDK1 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-PDK1 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PDPK1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.3 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-PDK-1 antibody shows detection of myc-tagged human PDK-1 at 60kDa in ~10 ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4¡ã C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG [H&L] (p/n 611-1302) and chemiluminescent detection is recommended. Other detection systems will yield similar results.
- Validation comment
- WB