TA319161

antibody from OriGene

Targeting: PDPK1 PDK1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319161

Provider

OriGene

Product name

Rabbit polyclonal anti-PDK1 antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal anti-PDK1 antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

PDPK1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

1.3 mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot using affinity purified anti-PDK-1 antibody shows detection of myc-tagged human PDK-1 at 60kDa in ~10 ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4¡ã C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG [H&L] (p/n 611-1302) and chemiluminescent detection is recommended. Other detection systems will yield similar results.

Validation comment

WB