PAB7926

antibody from Abnova Corporation

Targeting: PDPK1 PDK1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB7926

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB7926, RRID:AB_1710851

Product name

PDPK1 (phospho Y9) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of PDPK1.

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Nox4 NAD(P)H oxidase mediates Src-dependent tyrosine phosphorylation of PDK-1 in response to angiotensin II: role in mesangial cell hypertrophy and fibronectin expression.

Block K, Eid A, Griendling KK, Lee DY, Wittrant Y, Gorin Y
The Journal of biological chemistry 2008 Aug 29;283(35):24061-76

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Phosphoinositide-dependent phosphorylation of PDK1 regulates nuclear translocation.

Scheid MP, Parsons M, Woodgett JR
Molecular and cellular biology 2005 Mar;25(6):2347-63

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Pyk2- and Src-dependent tyrosine phosphorylation of PDK1 regulates focal adhesions.

Taniyama Y, Weber DS, Rocic P, Hilenski L, Akers ML, Park J, Hemmings BA, Alexander RW, Griendling KK
Molecular and cellular biology 2003 Nov;23(22):8019-29

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot of A-431 cells untreated (lanes 1) or treated with pervanadate (lanes 2, 3 & 4). Blots were probed with PDPK1 (phospho Y9) polyclonal antibody (Cat # PAB7926). The latter was used in the presence of no peptide (lane 2), phospho-PDK1 (Tyr-9) peptide (lane 3), or an unrelated phosphotyrosine peptide (lane 4).