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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- PAB10309 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10309, RRID:AB_1676219
- Product name
- PDPK1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PDPK1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Multiple phosphoinositide 3-kinase-dependent steps in activation of protein kinase B.
Involvement of Hsp90 in signaling and stability of 3-phosphoinositide-dependent kinase-1.
High resolution crystal structure of the human PDK1 catalytic domain defines the regulatory phosphopeptide docking site.
Identification of tyrosine phosphorylation sites on 3-phosphoinositide-dependent protein kinase-1 and their role in regulating kinase activity.
Scheid MP, Marignani PA, Woodgett JR
Molecular and cellular biology 2002 Sep;22(17):6247-60
Molecular and cellular biology 2002 Sep;22(17):6247-60
Involvement of Hsp90 in signaling and stability of 3-phosphoinositide-dependent kinase-1.
Fujita N, Sato S, Ishida A, Tsuruo T
The Journal of biological chemistry 2002 Mar 22;277(12):10346-53
The Journal of biological chemistry 2002 Mar 22;277(12):10346-53
High resolution crystal structure of the human PDK1 catalytic domain defines the regulatory phosphopeptide docking site.
Biondi RM, Komander D, Thomas CC, Lizcano JM, Deak M, Alessi DR, van Aalten DM
The EMBO journal 2002 Aug 15;21(16):4219-28
The EMBO journal 2002 Aug 15;21(16):4219-28
Identification of tyrosine phosphorylation sites on 3-phosphoinositide-dependent protein kinase-1 and their role in regulating kinase activity.
Park J, Hill MM, Hess D, Brazil DP, Hofsteenge J, Hemmings BA
The Journal of biological chemistry 2001 Oct 5;276(40):37459-71
The Journal of biological chemistry 2001 Oct 5;276(40):37459-71
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using PDPK1 polyclonal antibody (Cat # PAB10309) shows detection of myc-tagged human PDPK1 at 60 KDa in ~10 ug of a virus infected Sf9 cell lysate (arrow).The nitrocellulose membrane was probed overnight at 4°C with the primary antibody diluted 1 : 750 in 1% non-fat dry milk.HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection is recommended.
