M01159

antibody from Boster Biological Technology

Targeting: PDPK1 PDK1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

M01159

Provider

Boster Biological Technology

Product name

Anti-PDPK1/Pdk 1 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for PDK 1/PDPK1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat PDK 1/PDPK1 information: Molecular Weight: 63152 MW; Subcellular Localization: Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Cell junction, focal adhesion. Tyrosine phosphorylation seems to occur only at the cell membrane. Translocates to the cell membrane following insulin stimulation by a mechanism that involves binding to GRB14 and INSR. SRC and HSP90 promote its localization to the cell membrane. Its nuclear localization is dependent on its association with PTPN6 and its phosphorylation at Ser-396. Restricted to the nucleus in neuronal cells while in non-neuronal cells it is found in the cytoplasm. The Ser-241 phosphorylated form is distributed along the perinuclear region in neuronal cells while in non- neuronal cells it is found in both the nucleus and the cytoplasm. IGF1 transiently increases phosphorylation at Ser-241 of neuronal PDPK1, resulting in its translocation to other cellular compartments. The tyrosine-phosphorylated form colocalizes with PTK2B in foc

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

HOO-16

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.