ABIN361882

antibody from antibodies-online

Targeting: PDPK1 PDK1

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN361882

Provider

antibodies-online

Product name

anti-Pyruvate Dehydrogenase Kinase, Isozyme 1 (PDK1) (pSer241) antibody

Antibody type

Polyclonal

Antigen

Peptide sequence around phosphorylation site of pSer241 (A-N-S (p) -F-V) derived from Human PDK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Epitope

pSer241

Isotype

IgG

Vial size

100 μL

Concentration

1 mg/mL

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

References

Submitted references

Phosphoinositide-dependent phosphorylation of PDK1 regulates nuclear translocation.

Scheid MP, Parsons M, Woodgett JR
Molecular and cellular biology 2005 Mar;25(6):2347-63

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Nuclear translocation of 3'-phosphoinositide-dependent protein kinase 1 (PDK-1): a potential regulatory mechanism for PDK-1 function.

Lim MA, Kikani CK, Wick MJ, Dong LQ
Proceedings of the National Academy of Sciences of the United States of America 2003 Nov 25;100(24):14006-11

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Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3.

Sato S, Fujita N, Tsuruo T
The Journal of biological chemistry 2002 Oct 18;277(42):39360-7

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Insulin stimulates increased catalytic activity of phosphoinositide-dependent kinase-1 by a phosphorylation-dependent mechanism.

Chen H, Nystrom FH, Dong LQ, Li Y, Song S, Liu F, Quon MJ
Biochemistry 2001 Oct 2;40(39):11851-9