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- Antibody Data
- Antigen structure
- References [5]
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- Product number
- ABIN358390 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer396) antibody
- Antibody type
- Polyclonal
- Description
- Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- pSer396
- Vial size
- 0.4 mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Peroxisomal targeting as a tool for assaying potein-protein interactions in the living cell: cytokine-independent survival kinase (CISK) binds PDK-1 in vivo in a phosphorylation-dependent manner.
In vivo role of the PIF-binding docking site of PDK1 defined by knock-in mutation.
Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3.
Membrane localization of 3-phosphoinositide-dependent protein kinase-1 stimulates activities of Akt and atypical protein kinase C but does not stimulate glucose transport and glycogen synthesis in 3T3-L1 adipocytes.
PDK1-dependent activation of atypical PKC leads to degradation of the p21 tumour modifier protein.
Nilsen T, Slagsvold T, Skjerpen CS, Brech A, Stenmark H, Olsnes S
The Journal of biological chemistry 2004 Feb 6;279(6):4794-801
The Journal of biological chemistry 2004 Feb 6;279(6):4794-801
In vivo role of the PIF-binding docking site of PDK1 defined by knock-in mutation.
Collins BJ, Deak M, Arthur JS, Armit LJ, Alessi DR
The EMBO journal 2003 Aug 15;22(16):4202-11
The EMBO journal 2003 Aug 15;22(16):4202-11
Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3.
Sato S, Fujita N, Tsuruo T
The Journal of biological chemistry 2002 Oct 18;277(42):39360-7
The Journal of biological chemistry 2002 Oct 18;277(42):39360-7
Membrane localization of 3-phosphoinositide-dependent protein kinase-1 stimulates activities of Akt and atypical protein kinase C but does not stimulate glucose transport and glycogen synthesis in 3T3-L1 adipocytes.
Egawa K, Maegawa H, Shi K, Nakamura T, Obata T, Yoshizaki T, Morino K, Shimizu S, Nishio Y, Suzuki E, Kashiwagi A
The Journal of biological chemistry 2002 Oct 11;277(41):38863-9
The Journal of biological chemistry 2002 Oct 11;277(41):38863-9
PDK1-dependent activation of atypical PKC leads to degradation of the p21 tumour modifier protein.
Scott MT, Ingram A, Ball KL
The EMBO journal 2002 Dec 16;21(24):6771-80
The EMBO journal 2002 Dec 16;21(24):6771-80
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