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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- Ab121091 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- PDPK1 Mouse mAb
- Antibody type
- Monoclonal
- Description
- Application:WB: 1/500 - 1/2000. Predicted molecular weight: 63 kDa. IF/ICC: 1/200 - 1/1000 Flow: 1/200 - 1/400ELISA: 1/10000Protein FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 457-556- Isotype
- IgG
- Antibody clone number
- 3H3D9
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1 mg/ml
- Storage
- Shipped at 4¡C. Store at +4¡C short term (1-2 weeks). Upon delivery aliquot. Store at -20¡C long term. Avoid freeze / thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- PDPK1 Mouse mAb (Ab121091) - Western Blot All lanes: PDPK1 Mouse mAb (Ab121091) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 64 kDa Observed band size: 51- 64 kDa Exposure time: 113.7 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- PDPK1 Mouse mAb (Ab121091) - ICC/IF Immunofluorescence analysis of HeLa cells using PDPK1 Mouse mAb (Ab121091) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- PDPK1 Mouse mAb (Ab121091) - Flow Cytometry Flow cytometric analysis of HeLa cells using PDPK1 Mouse mAb (Ab121091) (green) and negative control (red).
