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ImmunocytochemistryAntibody data
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- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX16195 - Provider product page
- Provider
- GeneTex
- Product name
- TRAP1 antibody [TRAP1-6]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
Submitted references N-terminal functional domain of Gasdermin A3 regulates mitochondrial homeostasis via mitochondrial targeting.
Lin PH, Lin HY, Kuo CC, Yang LT
Journal of biomedical science 2015 Jun 24;22:44
Journal of biomedical science 2015 Jun 24;22:44
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- ICC/IF analysis of TRAP1 in NCI-H460 cells. Cells fixed with formaldehyde prior to staining. Cells were probed without (control) or with a TRAP1 monoclonal antibody(GTX16195) at a dilution of 1:200 overnight at 4¢J, washed with PBS and incubated with a dylight-488 conjugated secondary antibody. TRAP1 (green), F-actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Image were taken at 60X magnification.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of TRAP1 using GTX16195 visualized by Coomassie Blue staining
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a TRAP1 monoclonal antibody (GTX16195) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4¢XC in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
