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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [3]
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- Product number
- MA1-010 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRAP1 Monoclonal Antibody (TRAP1-6)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA1-010 detects tumor necrosis factor receptor-associated protein (TRAP1) from human tissues.
- Antibody clone number
- TRAP1-6
- Concentration
- 1 mg/mL
Submitted references Mitochondria are devoid of poly(ADP-ribose)polymerase-1, but harbor its product oligo(ADP-ribose).
Cytotoxicity of withaferin A in glioblastomas involves induction of an oxidative stress-mediated heat shock response while altering Akt/mTOR and MAPK signaling pathways.
A novel C-terminal HSP90 inhibitor KU135 induces apoptosis and cell cycle arrest in melanoma cells.
Involvement of tumor necrosis factor receptor-associated protein 1 (TRAP1) in apoptosis induced by beta-hydroxyisovalerylshikonin.
Hsp90 chaperone activity requires the full-length protein and interaction among its multiple domains.
The hsp90-related protein TRAP1 is a mitochondrial protein with distinct functional properties.
Köritzer J, Blenn C, Bürkle A, Beneke S
Journal of cellular biochemistry 2021 May;122(5):507-523
Journal of cellular biochemistry 2021 May;122(5):507-523
Cytotoxicity of withaferin A in glioblastomas involves induction of an oxidative stress-mediated heat shock response while altering Akt/mTOR and MAPK signaling pathways.
Grogan PT, Sleder KD, Samadi AK, Zhang H, Timmermann BN, Cohen MS
Investigational new drugs 2013 Jun;31(3):545-57
Investigational new drugs 2013 Jun;31(3):545-57
A novel C-terminal HSP90 inhibitor KU135 induces apoptosis and cell cycle arrest in melanoma cells.
Samadi AK, Zhang X, Mukerji R, Donnelly AC, Blagg BS, Cohen MS
Cancer letters 2011 Dec 22;312(2):158-67
Cancer letters 2011 Dec 22;312(2):158-67
Involvement of tumor necrosis factor receptor-associated protein 1 (TRAP1) in apoptosis induced by beta-hydroxyisovalerylshikonin.
Masuda Y, Shima G, Aiuchi T, Horie M, Hori K, Nakajo S, Kajimoto S, Shibayama-Imazu T, Nakaya K
The Journal of biological chemistry 2004 Oct 8;279(41):42503-15
The Journal of biological chemistry 2004 Oct 8;279(41):42503-15
Hsp90 chaperone activity requires the full-length protein and interaction among its multiple domains.
Johnson BD, Chadli A, Felts SJ, Bouhouche I, Catelli MG, Toft DO
The Journal of biological chemistry 2000 Oct 20;275(42):32499-507
The Journal of biological chemistry 2000 Oct 20;275(42):32499-507
The hsp90-related protein TRAP1 is a mitochondrial protein with distinct functional properties.
Felts SJ, Owen BA, Nguyen P, Trepel J, Donner DB, Toft DO
The Journal of biological chemistry 2000 Feb 4;275(5):3305-12
The Journal of biological chemistry 2000 Feb 4;275(5):3305-12
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of TRAP1 was achieved by transfecting HeLa with TRAP1 specific siRNAs (Silencer® select Product # s177). Western blot analysis (Fig. a) was performed using Whole cell extracts from the TRAP1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with TRAP1 Monoclonal Antibody (TRAP1-6) (Product # MA1-010, 1:2,000 ) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to TRAP1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-TRAP1 Monoclonal Antibody (TRAP1-6) (Product # MA1-010) and a 74kDa band corresponding to TRAP1 was observed across 4 cell lines. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), HEK-293 (Lane 3), K-562 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2,000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TRAP1 in NCI-H460 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a TRAP1 monoclonal antibody (Product # MA1-010) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). TRAP1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TRAP1 in PC-3-M cells using a TRAP1 monoclonal antibody (Product # MA1-010).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a TRAP1 monoclonal antibody (Product # MA1-010) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 6 Figure TRAP1 interacts with oADP-ribose in mitochondria. Mitochondria isolated from 10 8 HeLaS3 cells were immunoprecipitated using TRAP1 (75 kDa) antibody. 0.5% input, 5% of final wash, 25% post-elute, and 40% of eluted material of IP either with an unrelated IgG antibody (elute control IgG) or with TRAP1 antibody (elute) were probed for oADPR presence using LP96-10. Whereas the high molecular weight signal is visible in the input fraction, this is no longer detected in the TRAP1-IP elute, but the specific signal at 75 kDa (arrow). * denotes signals from mouse IgG used for IP. IP, immunoprecipitation; PARP, poly(ADP-ribose)polymerase
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of TRAP1 using Product # MA1-010 visualized by Coomassie Blue staining.
