PA5-27596

HSP75, HSP90L

Antibody data

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Validation data

Product number: PA5-27596
Provider: Invitrogen Antibodies
Product name: Anti-TRAP1 Polyclonal Antibody
Provider product page: Invitrogen Antibodies - PA5-27596
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: PA5-27596 targets TRAP1 in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples. The PA5-27596 immunogen is recombinant fragment corresponding to a region within amino acids 392 and 704 of Human TRAP1. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.66 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of TRAP1 using 50 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a TRAP1 polyclonal antibody (Product # PA5-27596) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of TRAP1 using 30 µg of A) HepG2 and B) MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a TRAP1 polyclonal antibody (Product # PA5-27596) at a dilution of 1:1500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: KD of TRAP1 was achieved by transfecting HeLa with TRAP1 specific siRNAs (Silencer® select Product # s177). Western blot analysis (Fig. a) was performed using membrane enriched cell extracts from the TRAP1 KD cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with TRAP1 Polyclonal Antibody (Product # PA5-27596, 1:1000 Dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to TRAP1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using TRAP1 Rabbit Polyclonal Antibody (Product # PA5-27596) and a 74kDa band corresponding to TRAP1 was observed across cell lines and tissue extracts tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep-G2 (Lane 2), HEK-293 (Lane 3), K-562 (Lane 4), T98G (Lane 5) and tissue extracts (30 µg lysate) of Mouse Liver (Lane 6), Rat Liver (Lane 7), Mouse Kidney (Lane 8) and Rat Kidney (Lane 9) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).