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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- BS-0530R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPAR gamma Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse stomach lysates probed with PPAR gamma Polyclonal Antibody, unconjugated (bs-0530R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sheep heart lysates probed with PPAR gamma Polyclonal Antibody, unconjugated (bs-0530R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 293T Cells probed with PPAR gamma Polyclonal Antibody, unconjugated (bs-0530R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody at 1:20000 for 60 minutes at 37°C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lane 1: Mouse Liver lysates; Lane 2: Mouse Heart lysates; Lane 3: Mouse Lung lysates probed with PPAR gamma Polyclonal Antibody, Unconjugated (bs-0530R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human skin psoriasis labeled with Rabbit Anti- PPARr gamma Polyclonal Antibody, Unconjugated (bs-0530R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded Mouse lung tissue labeled with Rabbit Anti- PPARr gamma Polyclonal Antibody, Unconjugated (bs-0530R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- U251 cells fixed with 70% ethanol (overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells were incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions and then probed with rabbit anti-PPARG Polyclonal Antibody, Unconjugated (bs-0530R) (green) at 1:100 for 30 minutes at room temperature, followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
