PA5-30244

antibody from Invitrogen Antibodies

Targeting: PKNOX1 PREP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30244

Provider

Invitrogen Antibodies

Product name

PKNOX1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2 nucleus.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PKNOX1 Polyclonal Antibody (Product # PA5-30244). Sample (30 µg of whole cell lysate). Lane A: HepG2. Lane B: HepG2 nucleus. 10% SDS PAGE. PKNOX1 Polyclonal Antibody (Product # PA5-30244) diluted at 1:2,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PKNOX1 showing staining in the nucleus of HepG2 cells. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a PKNOX1 polyclonal antibody (Product # PA5-30244) diluted at 1:1000. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PKNOX1 Polyclonal Antibody detects PKNOX1 protein at nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKNOX1 protein stained by PKNOX1 Polyclonal Antibody (Product # PA5-30244) diluted at 1:1,000. Blue: Hoechst 33342 staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PKNOX1 Polyclonal Antibody immunoprecipitates PKNOX1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of PKNOX1 protein by 2 µg of PKNOX1 antibody (Product # PA5-30244) 10% SDS-PAGE The immunoprecipitated PKNOX1 protein was detected by PKNOX1 antibody (Product # PA5-30244) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PKNOX1 Polyclonal Antibody immunoprecipitates PKNOX1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of PKNOX1 protein by 2 µg of PKNOX1 antibody (Product # PA5-30244) 10% SDS-PAGE The immunoprecipitated PKNOX1 protein was detected by PKNOX1 antibody (Product # PA5-30244) diluted at 1:1,000.