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Western blot
Immunocytochemistry
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Other assay [2]
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- Product number
- PA5-30244 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PKNOX1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2 nucleus. Predicted reactivity: Mouse (94%), Rat (94%), Rhesus Monkey (99%), Bovine (91%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PKNOX1 showing staining in the nucleus of HepG2 cells. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a PKNOX1 polyclonal antibody (Product # PA5-30244) diluted at 1:1000. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PKNOX1 Polyclonal Antibody detects PKNOX1 protein at nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKNOX1 protein stained by PKNOX1 Polyclonal Antibody (Product # PA5-30244) diluted at 1:1,000. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PKNOX1 Polyclonal Antibody immunoprecipitates PKNOX1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of PKNOX1 protein by 2 µg of PKNOX1 antibody (Product # PA5-30244) 10% SDS-PAGE The immunoprecipitated PKNOX1 protein was detected by PKNOX1 antibody (Product # PA5-30244) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PKNOX1 Polyclonal Antibody immunoprecipitates PKNOX1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of PKNOX1 protein by 2 µg of PKNOX1 antibody (Product # PA5-30244) 10% SDS-PAGE The immunoprecipitated PKNOX1 protein was detected by PKNOX1 antibody (Product # PA5-30244) diluted at 1:1,000.
