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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-29959 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal beta I Tubulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.4 ml
- Concentration
- 1.7 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta I Tubulin Antibody (86CT4.4.4) [NBP2-29959] - Western blot analysis of anti-TBB1 Antibody (NBP2-29959) in mouse brain tissue, Y79, CEM and 293 lysates (35ug/lane). TBB1 (arrow) was detected using the purified Mab.(1ug/ml)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta I Tubulin Antibody (86CT4.4.4) [NBP2-29959] - All lanes : Anti-TBB1 Antibody at 1:1000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: beta I Tubulin Antibody (86CT4.4.4) [NBP2-29959] - beta I Tubulin Antibody (86CT4.4.4) [NBP2-29959] - Confocal immunofluorescent analysis of TBB1 Antibody (NBP2-29959) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: beta I Tubulin Antibody (86CT4.4.4) [NBP2-29959] - Formalin-fixed and paraffin-embedded human brain reacted with TBB1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
