Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB8527 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal beta I Tubulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human beta-I Tubulin in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Calpain drives pyroptotic vimentin cleavage, intermediate filament loss, and cell rupture that mediates immunostimulation.
Davis MA, Fairgrieve MR, Den Hartigh A, Yakovenko O, Duvvuri B, Lood C, Thomas WE, Fink SL, Gale M Jr
Proceedings of the National Academy of Sciences of the United States of America 2019 Mar 12;116(11):5061-5070
Proceedings of the National Academy of Sciences of the United States of America 2019 Mar 12;116(11):5061-5070
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse beta-I Tubulin by Western Blot. Western blot shows lysates of human platelets and mouse platelets. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human beta-I Tubulin Monoclonal Antibody (Catalog # MAB8527) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta-I Tubulin at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.