MA5-31940

antibody from Invitrogen Antibodies

Targeting: TUBB1 dJ543J19.4

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-31940

Provider

Invitrogen Antibodies

Product name

TUBB1 Monoclonal Antibody (2A1A9)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

2A1A9

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Temporal resolution of gene derepression and proteome changes upon PROTAC-mediated degradation of BCL11A protein in erythroid cells.

Mehta S, Buyanbat A, Kai Y, Karayel O, Goldman SR, Seruggia D, Zhang K, Fujiwara Y, Donovan KA, Zhu Q, Yang H, Nabet B, Gray NS, Mann M, Fischer ES, Adelman K, Orkin SH
Cell chemical biology 2022 Aug 18;29(8):1273-1287.e8

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TUBB1 in human TUBB1 recombinant protein. Sample was incubated with TUBB1 monoclonal antibody (Product # MA5-31940) using a dilution of 1:500-1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TUBB1 in HEK293 (1) and TUBB1 cell lysate. Samples were incubated with TUBB1 monoclonal antibody (Product # MA5-31940) using a dilution of 1:500-1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TUBB1 in K562 (1), HepG2 (2), A431 (3), Jurkat (4), HeLa (5), NIH/3T3 (6), Cos7 (7) and PC12 (8) cell lysate. Samples were incubated with TUBB1 monoclonal antibody (Product # MA5-31940) using a dilution of 1:500-1:2000.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis of TUBB1 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with TUBB1 monoclonal antibody (Product # MA5-31940) using a dilution of 1:10,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of TUBB1 in Hela cells (green). Sample was incubated with TUBB1 monoclonal antibody (Product # MA5-31940) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TUBB1 in paraffin-embedded ovarian cancer tissue. Sample was incubated with TUBB1 monoclonal antibody (Product # MA5-31940) using a dilution of 1:200-1:1000 followed by DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TUBB1 in paraffin-embedded ovarian cancer tissue. Sample was incubated with TUBB1 monoclonal antibody (Product # MA5-31940) using a dilution of 1:200-1:1000 followed by DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of TUBB1 in A431 cells (green). Sample was incubated with TUBB1 monoclonal antibody (Product # MA5-31940) using a dilution of 1:200-1:400 followed by negative control (red).