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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-18915 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-18915, RRID:AB_1625867
- Product name
- Rabbit Polyclonal RanGAP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RanGAP1 Antibody [NBP1-18915] - Whole cell lysate from 293T and HeLa cells. RanGAP1 was also immunoprecipitated by rabbit anti-RanGAP1 antibody NBP1-18916.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RanGAP1 Antibody [NBP1-18915] - Detection of human and mouse RanGAP1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanGAP1 antibody NBP1-18915 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RanGAP1 Antibody [NBP1-18915] - Detection of human RanGAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanGAP1 antibody NBP1-18915 used for IP at 6 ug per reaction. RanGAP1 was also immunoprecipitated by another rabbit anti-RanGAP1 antibody. For blotting immunoprecipitated RanGAP1, NBP1-18915 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: RanGAP1 Antibody [NBP1-18915] - Detection of human RanGAP1 by immunohistochemistry. Samples: FFPE sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RanGAP1 Cat. No. NBP1-18915 (left) and another rabbit anti-RanGAP1 antibody (right) used at a dilution 1ug/ml. Detection: DAB
