H00006777-M02

antibody from Abnova Corporation

Targeting: STAT5B

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006777-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006777-M02, RRID:AB_519071

Product name

STAT5B monoclonal antibody (M02), clone 5D6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant STAT5B.

Antigen sequence

VYSKYYTPVPCESATAKAVDGYVKPQIKQVVPEFV
NASADAGGGSATYMDQAPSPAVCPQAHYNMYPQNP
DSVLDTDGDFDLEDTMDVARRVEELLGRPMDSQWI
PHAQS


Isotype

IgG

Antibody clone number

5D6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

STAT5B monoclonal antibody (M02), clone 5D6 Western Blot analysis of STAT5B expression in HeLa ( Cat # L013V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged STAT5B is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to STAT5B on HeLa cell. [antibody concentration 25 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to STAT5B on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 2 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol