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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab129471 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant STAT5b Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human STAT5b Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on.Application:WB: 1/500 Ð 1/1000IHC: 1/50-/100ICC/IF: 1/50-1/200Protein FunctionCarries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 600-786- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,5 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant STAT5b Antibody (Ab129471) - Western Blot All lanes: Recombinant STAT5b Antibody (Ab129471) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 90 kDa Observed band size: 87 kDa Exposure time: 12.2 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant STAT5b Antibody (Ab129471) - IF/ICC Immunocytochemistry analysis of STAT5b (green) in HeLa cells using Recombinant STAT5b Antibody (Ab129471), and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant STAT5b Antibody (Ab129471) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant STAT5b Antibody (Ab129471). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
