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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- R33860 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- AP2A1 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific AP2A1 antibody is suitable for use in Western blot/Immunocytochemistry/Immunohistochemistry/ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the AP2A1 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human frontal cortex lysate with AP2A1 antibody at 0.01ug/ml. Predicted molecular weight: ~108kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse brain lysate with AP2A1 antibody at 0.1ug/ml. The left panel shows the blot stained with Ponceau red for total protein (~5ug/lane).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC staining of methanol-fixed HeLa cells transfected with mouse Ap2a1 using AP2A1 antibody at 0.1ug/ml.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human brain (cortex) with AP2A1 antibody at 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
