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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB9935 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9935, RRID:AB_1672111
- Product name
- AP2A1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of AP2A1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification and regulation of tissue-specific cis-acting elements associated with the human AP-2alpha gene.
Cloning, physical mapping and structural characterization of the human alpha(A)-adaptin gene.
Adaptins: the final recount.
Zhang J, Williams T
Developmental dynamics : an official publication of the American Association of Anatomists 2003 Oct;228(2):194-207
Developmental dynamics : an official publication of the American Association of Anatomists 2003 Oct;228(2):194-207
Cloning, physical mapping and structural characterization of the human alpha(A)-adaptin gene.
Scorilas A, Levesque MA, Ashworth LK, Diamandis EP
Gene 2002 May 1;289(1-2):191-9
Gene 2002 May 1;289(1-2):191-9
Adaptins: the final recount.
Boehm M, Bonifacino JS
Molecular biology of the cell 2001 Oct;12(10):2907-20
Molecular biology of the cell 2001 Oct;12(10):2907-20
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using AP2A1 polyclonal antibody (Cat # PAB9935) shows detection of a band just below 100 KDa correspond-ing to Human AP2A1 in a various preparations.Lane 1 - HeLa nuclear extract.Lane 2 - HeLa.Lane 3 - 293.Lane 4 - A-431.Lane 5 - Jurkat whole cell lysates.In lanes 6-10 the antibody was preincubated with 1 ug/mL of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A1.Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of AP2A1 polyclonal antibody.Detection occurred using a 1 : 5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature.A chemiluminescence system was used for signal detection using a 60-sec exposure time.
