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- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- EB11875 - Provider product page
- Provider
- Everest Biotech
- Product name
- Goat anti-AP2A1 (aa706-727) Antibody
- Antibody type
- Polyclonal
- Description
- Goat anti-AP2A1 (aa706-727) Antibody
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
C-ELEPPAPESPMALLADPAPAAD- Epitope
- internal region
- Isotype
- IgG
- Vial size
- 100µg specific antibody in 200µl
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing.
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Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB11875 (0.01µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB11875 (0.1µg/ml) staining of Mouse Brain lysate (~5µg protein in SDSPAGE buffer). The left panel shows the same blot stained with Ponceau red for total protein stain as the loading control before labelling. Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK
Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB11875 (0.1µg/ml) staining of methanol-fixed HeLa cells with stably expressing Mouse Ap2a1. Primary incubation was 1 hour. Detected by Alexa Fluor 594. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK
