Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS1962 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- AKAP 5 (E7) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to the N-terminal of Human AKAP 5.
- Description
- AKAPs (A-kinase anchoring proteins), as their name implies, are a family of scaffolding proteins that bind regulatory subunits of Protein Kinase A (PKA) thus localizing PKA activity to distinct regions of the cell. Beyond a common amphipathic alpha helix that is responsible for recruiting the PKA regulatory subunit (RIÏ«, RIIÏ«, RIÉÇ, or RIIÉÇ), individual AKAPs contain additional domains responsible for the recrutiment of additional signaling proteins (phosphodiesterases, phosphatases, cytoskeletal components, other kinase, etc.) or restricting AKAP to a specific subcellular location. AKAP5 (also known as P75, AKAP75, or AKAP79) is predominantly expressed in neuronal tissues and cells where it serves to localize type II PKA to post-synaptic densities. AKAP5 specifically binds to the regulatory subunit of PKAIIÉÇ, anchoring the enzyme to the plasma membrane and sites of cytoskeletal/membrane junctions. The other binding domains of AKAP5 have been shown to interact with calmodulin, PP2B, and calcineurin suggesting that AKAP5 may act to coordinate the cAMP- and Ca2+-sensing pathways in various cell types.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of AKAP 5 (E7) pAb at 1:500 dilutionLane1:HEK293T cell lysateLane2:NIH-3T3 cell lysateLane3:H9C2 cell lysate