11256-1-AP

antibody from Proteintech Group

Targeting: ANXA2 ANX2, ANX2L4, CAL1H, LIP2, LPC2D

Provider product page for 11256-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [64]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [1]

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Product number: 11256-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#11256-1-AP, RRID:AB_2057311
Product name: Annexin A2 antibody
Antibody type: Polyclonal
Description: KD/KO validated Annexin A2 antibody (Cat. #11256-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

ANXA2 protein structure - 11256-1-AP shown in red.

Submitted references Exosomes Derived from Meningitic Escherichia coli-Infected Brain Microvascular Endothelial Cells Facilitate Astrocyte Activation.

Yang R, Qu X, Zhi S, Wang J, Fu J, Tan C, Chen H, Wang X
Molecular neurobiology 2024 Sep;61(9):7195-7210

Squalene monooxygenase facilitates bladder cancer development in part by regulating PCNA.

Zhu C, Fang X, Liu X, Jiang C, Ren W, Huang W, Jiang Y, Wang D
Biochimica et biophysica acta. Molecular cell research 2024 Mar;1871(3):119681

Opportunistic pathogen Porphyromonas gingivalis targets the LC3B-ceramide complex and mediates lethal mitophagy resistance in oral tumors.

Sheridan M, Chowdhury N, Wellslager B, Oleinik N, Kassir MF, Lee HG, Engevik M, Peterson Y, Pandruvada S, Szulc ZM, Yilmaz Ö, Ogretmen B
iScience 2024 Jun 21;27(6):109860

The lncRNA GAS5 upregulates ANXA2 to mediate the macrophage inflammatory response during atherosclerosis development.

Xue Y, Hu Y, Yu S, Zhu W, Liu L, Luo M, Luo S, Shen J, Huang L, Liu J, Lv D, Zhang W, Wang J, Li X
Heliyon 2024 Jan 30;10(2):e24103

Modulation of prostaglandin transport activity of SLCO2A1 by annexin A2 and S100A10.

Nakamura Y, Ito MA, Hoshino Y, Matsuoka I, Okada T, Okada Y, Nakanishi T
American journal of physiology. Cell physiology 2024 Apr 1;326(4):C1042-C1053

Lysyl oxidase-like 4 exerts an atypical role in breast cancer progression that is dependent on the enzymatic activity that targets the cell-surface annexin A2.

Komalasari NLGY, Tomonobu N, Kinoshita R, Chen Y, Sakaguchi Y, Gohara Y, Jiang F, Yamamoto KI, Murata H, Ruma IMW, Sumardika IW, Zhou J, Yamauchi A, Kuribayashi F, Inoue Y, Toyooka S, Sakaguchi M
Frontiers in oncology 2023;13:1142907

Circular RNA EIF3I promotes papillary thyroid cancer progression by interacting with AUF1 to increase Cyclin D1 production.

Yao X, Liu H, Wang Z, Lu F, Chen W, Feng Q, Miao Y, Zhang J, Wang Y, Chen Y, Xue L, Liu Y, Chen L, Zhang Q
Oncogene 2023 Oct;42(43):3206-3218

ANNEXIN A2 FACILITATES NEOVASCULARIZATION TO PROTECT AGAINST MYOCARDIAL INFARCTION INJURY VIA INTERACTING WITH MACROPHAGE YAP AND ENDOTHELIAL INTEGRIN Β3.

Zhang Y, Wang Y, Li J, Li C, Liu W, Long X, Wang Z, Zhao R, Ge J, Shi B
Shock (Augusta, Ga.) 2023 Oct 1;60(4):573-584

Inhibition of ANXA2 activity attenuates epileptic susceptibility and GluA1 phosphorylation.

Ma L, Wu Q, Yuan J, Wang Y, Zhang P, Liu Q, Tan D, Liang M, Chen Y
CNS neuroscience & therapeutics 2023 Nov;29(11):3644-3656

LppA is a novel plasminogen receptor of Mycoplasma bovis that contributes to adhesion by binding the host extracellular matrix and Annexin A2.

Liu S, Li Z, Lan S, Hao H, Jin X, Liang J, Baz AA, Yan X, Gao P, Chen S, Chu Y
Veterinary research 2023 Nov 17;54(1):107

Downregulation of Calreticulin and Annexin A2 Expression in Acquired Middle Ear Cholesteatoma by 2-DE Analysis.

Chang NC, Wu YJ, Wang LF, Chan LP, Chai CY, Chen Ms WT, Tsai SM, Chien CY, Ho KY
The Annals of otology, rhinology, and laryngology 2023 Jun;132(6):684-691

Development of a Novel Circulating Autoantibody Biomarker Panel for the Identification of Patients with 'Actionable' Pulmonary Nodules.

Auger C, Moudgalya H, Neely MR, Stephan JT, Tarhoni I, Gerard D, Basu S, Fhied CL, Abdelkader A, Vargas M, Hu S, Hulett T, Liptay MJ, Shah P, Seder CW, Borgia JA
Cancers 2023 Apr 12;15(8)

Baicalin Attenuates Continuous Activation of β-Catenin Induced by Lipopolysaccharide (LPS) and Depression Complicated by Infertility in Male Rats.

Fan R, Dai F, Zhang C, Zhou L, Qiu X, Li Y
Evidence-based complementary and alternative medicine : eCAM 2022;2022:2112359

FYN regulates cell adhesion at the blood-testis barrier and the apical ectoplasmic specialization via its effect on Arp3 in the mouse testis.

Yang Y, Yao M, Zeng J, Zheng D, Li Q, Ni Y, Xiao X
Frontiers in immunology 2022;13:915274

Astrocyte immunometabolic regulation of the tumour microenvironment drives glioblastoma pathogenicity.

Perelroizen R, Philosof B, Budick-Harmelin N, Chernobylsky T, Ron A, Katzir R, Shimon D, Tessler A, Adir O, Gaoni-Yogev A, Meyer T, Krivitsky A, Shidlovsky N, Madi A, Ruppin E, Mayo L
Brain : a journal of neurology 2022 Sep 14;145(9):3288-3307

HK1 from hepatic stellate cell-derived extracellular vesicles promotes progression of hepatocellular carcinoma.

Chen QT, Zhang ZY, Huang QL, Chen HZ, Hong WB, Lin T, Zhao WX, Wang XM, Ju CY, Wu LZ, Huang YY, Hou PP, Wang WJ, Zhou D, Deng X, Wu Q
Nature metabolism 2022 Oct;4(10):1306-1321

STARD3NL inhibits the osteogenic differentiation by inactivating the Wnt/β-catenin pathway via binding to Annexin A2 in osteoporosis.

Xu Y, Bao X, Chen X, Wu P, Chen S, Zhang B, Ma J, Xu G, Ma D
Journal of cellular and molecular medicine 2022 Mar;26(5):1643-1655

AMG487 inhibits PRRSV replication and ameliorates lung injury in pig lung xenografts by down-regulating the expression of ANXA2.

Liu J, Yao L, Huang S, Wang B, Li L, Li L, Gu W, Xiao S, Liu G
Antiviral research 2022 Jun;202:105314

LINC00941 promotes pancreatic cancer malignancy by interacting with ANXA2 and suppressing NEDD4L-mediated degradation of ANXA2.

Wang J, He Z, Liu X, Xu J, Jiang X, Quan G, Jiang J
Cell death & disease 2022 Aug 18;13(8):718

5α-Epoxyalantolactone Inhibits Metastasis of Triple-Negative Breast Cancer Cells by Covalently Binding a Conserved Cysteine of Annexin A2.

Wei M, Zhou Y, Li C, Yang Y, Liu T, Liu Y, Wei Y, Liu N, Liu S, Wang Q, Cao S, Sun Y, Sheng P, Lu C, Yang C, Liu X, Yang G
Journal of medicinal chemistry 2021 Sep 9;64(17):12537-12547

Interaction of FLNA and ANXA2 promotes gefitinib resistance by activating the Wnt pathway in non-small-cell lung cancer.

Cheng L, Tong Q
Molecular and cellular biochemistry 2021 Oct;476(10):3563-3575

Effect and mechanism of action of SLP-2 on the apoptosis and autophagy of gastric cancer cells.

Yang S, Huang Y, Zhang H, Wang F, Shao L, Wang X
Oncology letters 2021 Oct;22(4):707

LINC01133 promotes hepatocellular carcinoma progression by sponging miR-199a-5p and activating annexin A2.

Yin D, Hu ZQ, Luo CB, Wang XY, Xin HY, Sun RQ, Wang PC, Li J, Fan J, Zhou ZJ, Zhou J, Zhou SL
Clinical and translational medicine 2021 May;11(5):e409

Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.

Asada K, Sakaue F, Nagata T, Zhang JC, Yoshida-Tanaka K, Abe A, Nawa M, Nishina K, Yokota T
Nucleic acids research 2021 May 21;49(9):4864-4876

Expressions of P-Glycoprotein, Multidrug Resistance Protein 1 and Annexin A2 as Predictive Factors for Intravesical Recurrence of Bladder Cancer after the Initial Transurethral Resection and Immediate Single Intravesical Instillation of Adriamycin.

Sawazaki H, Ito K, Asano T, Kuroda K, Horiguchi A, Tsuda H, Asano T
Asian Pacific journal of cancer prevention : APJCP 2021 May 1;22(5):1459-1466

Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.

Qu H, Gong X, Liu X, Zhang R, Wang Y, Huang B, Zhang L, Zheng H, Zheng Y
Diabetes 2021 Jun;70(6):1372-1387

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Tan X, Banerjee P, Shi L, Xiao GY, Rodriguez BL, Grzeskowiak CL, Liu X, Yu J, Gibbons DL, Russell WK, Creighton CJ, Kurie JM
Science advances 2021 Jun;7(25)

YAP-dependent proliferation by a small molecule targeting annexin A2.

Shalhout SZ, Yang PY, Grzelak EM, Nutsch K, Shao S, Zambaldo C, Iaconelli J, Ibrahim L, Stanton C, Chadwick SR, Chen E, DeRan M, Li S, Hull M, Wu X, Chatterjee AK, Shen W, Camargo FD, Schultz PG, Bollong MJ
Nature chemical biology 2021 Jul;17(7):767-775

CD147 deficiency in T cells prevents thymic involution by inhibiting the EMT process in TECs in the presence of TGFβ.

Chen R, Wang K, Feng Z, Zhang MY, Wu J, Geng JJ, Chen ZN
Cellular & molecular immunology 2021 Jan;18(1):171-181

TRIM21-regulated Annexin A2 plasma membrane trafficking facilitates osteosarcoma cell differentiation through the TFEB-mediated autophagy.

Zhang HT, Zeng Q, Wu B, Lu J, Tong KL, Lin J, Liu QY, Xu L, Yang J, Liu X, Liu W, Zhang YF, Lian Q, Liu L, Gao X
Cell death & disease 2021 Jan 6;12(1):21

Exosomal ANXA2 derived from ovarian cancer cells regulates epithelial-mesenchymal plasticity of human peritoneal mesothelial cells.

Gao L, Nie X, Gou R, Hu Y, Dong H, Li X, Lin B
Journal of cellular and molecular medicine 2021 Dec;25(23):10916-10929

A novel cystathionine γ-lyase inhibitor, I194496, inhibits the growth and metastasis of human TNBC via downregulating multiple signaling pathways.

Liu Y, Wang L, Zhang X, Deng Y, Pan L, Li H, Shi X, Wang T
Scientific reports 2021 Apr 26;11(1):8963

Plasma Exosomes Derived From Patients With End-Stage Renal Disease and Renal Transplant Recipients Have Different Effects on Vascular Calcification.

Lin X, Zhu T, Xu F, Zhong JY, Li F, Shan SK, Wu F, Guo B, Zheng MH, Wang Y, Xu QS, Liao XB, Lu HY, Xie XB, Yuan LQ
Frontiers in cell and developmental biology 2020;8:618228

Matrisome Provides a Supportive Microenvironment for Skin Functions of Diverse Species.

Liu B, Zhang S, Wang W, Yun Z, Lv L, Chai M, Wu Z, Zhu Y, Ma J, Leng L
ACS biomaterials science & engineering 2020 Oct 12;6(10):5720-5733

(20S)G-Rh2 Inhibits NF-κB Regulated Epithelial-Mesenchymal Transition by Targeting Annexin A2.

Wang YS, Li H, Li Y, Zhang S, Jin YH
Biomolecules 2020 Mar 31;10(4)

Transgelin-2 contributes to proliferation and progression of hepatocellular carcinoma via regulating Annexin A2.

Shi J, Ren M, She X, Zhang Z, Zhao Y, Han Y, Lu D, Lyu L
Biochemical and biophysical research communications 2020 Mar 12;523(3):632-638

Enterovirus A71 capsid protein VP1 increases blood-brain barrier permeability and virus receptor vimentin on the brain endothelial cells.

Wang W, Sun J, Wang N, Sun Z, Ma Q, Li J, Zhang M, Xu J
Journal of neurovirology 2020 Feb;26(1):84-94

Proteomic Analysis Reveals Proteins and Pathways Associated with Lactation in Bovine Mammary Epithelial Cell-Derived Exosomes.

Zhang M, Ma Z, Li R, Guo S, Qiu Y, Gao X
Journal of proteome research 2020 Aug 7;19(8):3211-3219

Rosmarinic acid exerts a neuroprotective effect on spinal cord injury by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways.

Ma Z, Lu Y, Yang F, Li S, He X, Gao Y, Zhang G, Ren E, Wang Y, Kang X
Toxicology and applied pharmacology 2020 Apr 19;397:115014

NUDT21 inhibits bladder cancer progression through ANXA2 and LIMK2 by alternative polyadenylation.

Xiong M, Chen L, Zhou L, Ding Y, Kazobinka G, Chen Z, Hou T
Theranostics 2019;9(24):7156-7167

Annexin A2 is involved in activation of extracellular signal-regulated kinase upon endothelin-1 stimulation.

Mazaki Y, Higashi T, Horinouchi T, Miwa S
Biochemical and biophysical research communications 2019 Mar 26;511(1):69-72

Compounds targeting YadC of uropathogenic Escherichia coli and its host receptor annexin A2 decrease bacterial colonization in bladder.

Li X, Pei G, Zhang L, Cao Y, Wang J, Yu L, Dianjun W, Gao S, Zhang ZS, Yao Z, Wang Q
EBioMedicine 2019 Dec;50:23-33

Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer.

Xu S, Zhan M, Jiang C, He M, Yang L, Shen H, Huang S, Huang X, Lin R, Shi Y, Liu Q, Chen W, Mohan M, Wang J
Nature communications 2019 Dec 2;10(1):5492

NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

Huang D, Chen J, Yang L, Ouyang Q, Li J, Lao L, Zhao J, Liu J, Lu Y, Xing Y, Chen F, Su F, Yao H, Liu Q, Su S, Song E
Nature immunology 2018 Oct;19(10):1112-1125

TRIM65 supports bladder urothelial carcinoma cell aggressiveness by promoting ANXA2 ubiquitination and degradation.

Wei WS, Chen X, Guo LY, Li XD, Deng MH, Yuan GJ, He LY, Li YH, Zhang ZL, Jiang LJ, Chen RX, Ma XD, Wei S, Ma NF, Liu ZW, Luo JH, Zhou FJ, Xie D
Cancer letters 2018 Oct 28;435:10-22

Annexin A2 positively regulates milk synthesis and proliferation of bovine mammary epithelial cells through the mTOR signaling pathway.

Zhang M, Chen D, Zhen Z, Ao J, Yuan X, Gao X
Journal of cellular physiology 2018 Mar;233(3):2464-2475

Mechanism of endogenous digitalis-like factor‑induced vascular endothelial cell damage in patients with severe preeclampsia.

Peng M, Yang M, Ding Y, Yu L, Deng Y, Lai W, Hu Y
International journal of molecular medicine 2018 Feb;41(2):985-994

Expression of annexin II and stromal tenascin C promotes epithelial to mesenchymal transition and correlates with distant metastasis in pancreatic cancer.

Yoneura N, Takano S, Yoshitomi H, Nakata Y, Shimazaki R, Kagawa S, Furukawa K, Takayashiki T, Kuboki S, Miyazaki M, Ohtsuka M
International journal of molecular medicine 2018 Aug;42(2):821-830

Heterotetrameric annexin A2/S100A10 (A2t) is essential for oncogenic human papillomavirus trafficking and capsid disassembly, and protects virions from lysosomal degradation.

Taylor JR, Fernandez DJ, Thornton SM, Skeate JG, Lühen KP, Da Silva DM, Langen R, Kast WM
Scientific reports 2018 Aug 3;8(1):11642

Label-free proteomic analysis of placental proteins during Toxoplasma gondii infection.

Jiao F, Zhang D, Jiang M, Mi J, Liu X, Zhang H, Hu Z, Xu X, Hu X
Journal of proteomics 2017 Jan 6;150:31-39

Annexin A2 Enhances Complement Activation by Inhibiting Factor H.

Renner B, Tong HH, Laskowski J, Jonscher K, Goetz L, Woolaver R, Hannan J, Li YX, Hourcade D, Pickering MC, Holers VM, Thurman JM
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1355-65

Nicotine elevates sperm motility and induces Pfn1 promoter hypomethylation in mouse testis.

Dai J, Zhan C, Xu W, Wang Z, Nie D, Zhao X, Zhang D, Gu Y, Wang L, Chen Z, Qiao Z
Andrology 2015 Sep;3(5):967-78

Annexin A2 as a target endothelial cell membrane autoantigen in Behçet's disease.

Chen P, Yan H, Tian Y, Xun Y, Shi L, Bao R, Zhang H, Chen G, Yang C, Sun S, Wang Y, Liu L, Zhou Y, Zhang C, Wang X, Wen Y, Bian Y, Du H
Scientific reports 2015 Feb 2;5:8162

The expression of annexin II and Lewis y antigen in ovarian epithelial tumors and the correlation between them.

Zhuang H, Tan M, Liu J, Li X, Gao J, Hu Z, Deng L, Zhu L, Lin B
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Apr;36(4):2343-9

Human epididymis protein 4 in association with Annexin II promotes invasion and metastasis of ovarian cancer cells.

Zhuang H, Tan M, Liu J, Hu Z, Liu D, Gao J, Zhu L, Lin B
Molecular cancer 2014 Nov 1;13:243

A panel of tumor markers, calreticulin, annexin A2, and annexin A3 in upper tract urothelial carcinoma identified by proteomic and immunological analysis.

Lu CM, Lin JJ, Huang HH, Ko YC, Hsu JL, Chen JC, Din ZH, Wu YJ
BMC cancer 2014 May 23;14:363

The interaction between host Annexin A2 and viral Nsp9 is beneficial for replication of porcine reproductive and respiratory syndrome virus.

Li J, Guo D, Huang L, Yin M, Liu Q, Wang Y, Yang C, Liu Y, Zhang L, Tian Z, Cai X, Yu L, Weng C
Virus research 2014 Aug 30;189:106-13

The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis.

Zhu Z, Xu W, Dai J, Chen X, Zhao X, Fang P, Yang F, Tang M, Wang Z, Wang L, Ma D, Qaio Z
The international journal of biochemistry & cell biology 2013 Mar;45(3):571-82

Clinical significance of annexin II expression in human non-small cell lung cancer.

Jia JW, Li KL, Wu JX, Guo SL
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2013 Jun;34(3):1767-71

Comparative proteomics of Hirschsprung's disease.

Fan Y, Wang L, Zhang Y, Huang T, Li H, Gu H, Wang W, Yuan Z
Journal of proteomics 2013 Jun 12;84:176-84

Proteomic analysis of the differences in orbital protein expression in thyroid orbitopathy.

Cheng KC, Huang HH, Hung CT, Chen CC, Wu WC, Suen JL, Chen KJ, Wu YJ, Chang CH
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2013 Dec;251(12):2777-87

Proteomic profiling of the 11-dehydrosinulariolide-treated oral carcinoma cells Ca9-22: effects on the cell apoptosis through mitochondrial-related and ER stress pathway.

Liu CI, Wang RY, Lin JJ, Su JH, Chiu CC, Chen JC, Chen JY, Wu YJ
Journal of proteomics 2012 Oct 22;75(18):5578-89

Identification of human host proteins contributing to H5N1 influenza virus propagation by membrane proteomics.

Liu C, Zhang A, Guo J, Yang J, Zhou H, Chen H, Jin M
Journal of proteome research 2012 Nov 2;11(11):5396-405

Isolation and characterization of cytoplasmic cofilin-actin rods.

Minamide LS, Maiti S, Boyle JA, Davis RC, Coppinger JA, Bao Y, Huang TY, Yates J, Bokoch GM, Bamburg JR
The Journal of biological chemistry 2010 Feb 19;285(8):5450-60

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 11256-1-AP(ANXA2 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using ANXA2 antibody 11256-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-ANXA2 (IP:11256-1-AP, 3ug; Detection:11256-1-AP 1:500) with HeLa cells lysate 3000ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 11256-1-AP(ANXA2 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue