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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- AP7420a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP7420a, RRID:AB_1277905
- Product name
- ANXA2 Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of ANXA2 (arrow) using rabbit polyclonal ANXA2 Antibody (N-term) (Cat.#AP7420a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ANXA2 gene.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-ANXA2 Antibody (N-term) (Cat.#AP7420a) in mouse lung tissue lysates (35ug/lane). ANXA2(arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "ANXA2 Antibody (N-term) (Cat. #AP7420a) western blot analysis in 293,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the ANXA2 antibody detected the ANXA2 protein (arrow)."
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody (N-term) (Cat.#AP7420a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:10~50
