PA5-27085

antibody from Invitrogen Antibodies

Targeting: ANXA2 ANX2, ANX2L4, CAL1H, LIP2, LPC2D

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27085

Provider

Invitrogen Antibodies

Product name

Annexin A2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: A549, HeLa, HCT116, human ANXA2-transfected 293T.

Concentration

0.75 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Annexin A2 was performed by separating 30 µg of non-transfected (-) and human Annexin I or Annexin II-transfected (+) whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Annexin A2 Polyclonal Antibody (Product # PA5-27085) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Annexin A2 Polyclonal Antibody detects ANXA2 protein by western blot analysis. A. 30 µg A549 whole cell lysate/extract. B. 30 µg HCT116 whole cell lysate/extract.10% SDS-PAGE. Annexin A2 Polyclonal Antibody (Product # PA5-27085) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Annexin A2 Polyclonal Antibody(Product # PA5-27085) and a 40kDa band corresponding to Annexin A2 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of A549 (Lane 1), HCT 116 (Lane 2), HeLa (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 Dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Annexin II in paraformaldehyde-fixed HeLa cells using an Annexin II polyclonal antibody (Product # PA5-27085) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue, using ANXA2 (Product # PA5-27085) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue, using ANXA2 (Product # PA5-27085) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.