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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27566 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Annexin A2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HCT116, HeLa, GL261, NIH-3T3, C2C12, Rat2, human ANXA2-transfected 293T.
- Concentration
- 0.14 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Annexin II using A) 30 µg A549 whole cell lysate and B) 30 µg HCT116 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Annexin II polyclonal antibody (Product # PA5-27566) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Annexin A2 was performed by separating 30 µg of non-transfected (-) and human Annexin I or Annexin II-transfected (+) whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Annexin A2 Polyclonal Antibody (Product # PA5-27566) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Annexin A2 Polyclonal Antibody (Product # PA5-27566). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A2 Polyclonal Antibody (Product # PA5-27566) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Annexin A2 Polyclonal Antibody detects ANXA2 protein by Western blot analysis. A. 30 µg GL261 whole cell lysate/extract. B. 30 µg NIH-3T3 whole cell lysate/extract. C. 30 µg C2C12 whole cell lysate/extract.10 % SDS-PAGE. Annexin A2 Polyclonal Antibody (Product # PA5-27566) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Annexin A2 Polyclonal Antibody (Product # PA5-27566). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A2 Polyclonal Antibody (Product # PA5-27566) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Annexin A2 Polyclonal Antibody detects ANXA2 protein by Western blot analysis. A. 30 µg Rat2 whole cell lysate/extract.10 % SDS-PAGE. Annexin A2 Polyclonal Antibody (Product # PA5-27566) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Annexin II in paraformaldehyde-fixed A431 cells using an Annexin II polyclonal antibody (Product # PA5-27566) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Annexin A2 Polyclonal Antibody (Product # PA5-27566) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
