PA5-27566

antibody from Invitrogen Antibodies

Targeting: ANXA2 ANX2, ANX2L4, CAL1H, LIP2, LPC2D

Antibody data

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Validation data

Product number: PA5-27566 - Provider product page
Provider: Invitrogen Antibodies
Product name: Annexin A2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: A549, HCT116, HeLa, GL261, NIH-3T3, C2C12, Rat2, human ANXA2-transfected 293T. Predicted reactivity: Mouse (98%), Rat (94%), Xenopus laevis (81%), Dog (100%), Pig (100%), Chicken (90%), Sheep (99%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.14 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ANXA2 protein structure - PA5-27566 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Annexin II using A) 30 µg A549 whole cell lysate and B) 30 µg HCT116 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Annexin II polyclonal antibody (Product # PA5-27566) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Annexin A2 was performed by separating 30 µg of non-transfected (-) and human Annexin I or Annexin II-transfected (+) whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Annexin A2 Polyclonal Antibody (Product # PA5-27566) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Annexin A2 Polyclonal Antibody (Product # PA5-27566). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A2 Polyclonal Antibody (Product # PA5-27566) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Annexin A2 Polyclonal Antibody detects ANXA2 protein by Western blot analysis. A. 30 µg GL261 whole cell lysate/extract. B. 30 µg NIH-3T3 whole cell lysate/extract. C. 30 µg C2C12 whole cell lysate/extract.10 % SDS-PAGE. Annexin A2 Polyclonal Antibody (Product # PA5-27566) dilution: 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Annexin A2 Polyclonal Antibody (Product # PA5-27566). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A2 Polyclonal Antibody (Product # PA5-27566) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Annexin A2 Polyclonal Antibody detects ANXA2 protein by Western blot analysis. A. 30 µg Rat2 whole cell lysate/extract.10 % SDS-PAGE. Annexin A2 Polyclonal Antibody (Product # PA5-27566) dilution: 1:1,000.